1NFH
Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793, 0.9595, 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.590, 86.590, 81.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.247 |
| Rwork | 0.237 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.084 | 0.285 |
| Number of reflections | 9156 | |
| <I/σ(I)> | 21.8 | 6 |
| Completeness [%] | 96.6 * | 97.7 |
| Redundancy | 6.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | iso-propanol, KCl, MgCl2, Na-cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 15 (%) | |
| 3 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.0 |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | 25 (mM) |
