1NEZ

The Crystal Structure of a TL/CD8aa Complex at 2.1A resolution:Implications for Memory T cell Generation, Co-receptor Preference and Affinity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	160
Wavelength(s)	1.01
Spacegroup name	P 31 2 1
Unit cell lengths	77.018, 77.018, 176.045
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.217
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011 *
RMSD bond angle	1.643 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100	2.100 *
Rmerge	0.071	0.500 *
Total number of observations	171093 *
Number of reflections	35619
<I/σ(I)>	9.7
Completeness [%]	98.9	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	2.0M ammonium sulfate, 2.0% PEG 400, 5% DMSO, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	PEG400	2.0 (%)
4	1	reservoir	DMSO	5 (%)
5	1	reservoir	HEPES	0.1 (M)	pH7.5