1NDZ
Crystal Structure of Adenosine Deaminase Complexed with FR235999
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-30 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.836 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.060, 78.060, 136.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
Rwork | 0.210 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.037 * |
RMSD bond angle | 4.600 * |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.126 |
Number of reflections | 28210 | |
Completeness [%] | 96.3 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 293 | Kinoshita, T., (1999) Acta Crystallogr.,Sect., D55, 2031. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.0-2.2 (M) | |
2 | 1 | reservoir | PEG400 | 2 (%(v/v)) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7. |
4 | 1 | drop | protein | 20 (mg/ml) | |
5 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
6 | 1 | drop | purine riboside | 10 (mM) |