1NDK

X-RAY STRUCTURE OF NUCLEOSIDE DIPHOSPHATE KINASE

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	72.700, 72.700, 107.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 * - 2.200
R-factor	0.203
Rwork	0.203
RMSD bond length	0.010
RMSD bond angle	1.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.046 *
Total number of observations	82562 *
Number of reflections	9012 *
Completeness [%]	99.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	drop	Tris-HCl	30 (mM)
3	1	drop	ammonium sulfate	1 (M)
4	1	drop		20 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	reservoir	ammonium sulfate	2 (M)