1NDJ
Streptavidin Mutant Y43F with Biotin at 1.81A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.300, 98.400, 52.800 |
Unit cell angles | 90.00, 112.90, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 * |
R-factor | 0.2273 |
Rwork | 0.222 |
R-free | 0.31200 |
Structure solution method | ISOMORPHOUS |
Starting model (for MR) | 1swe |
RMSD bond length | 0.006 |
RMSD bond angle | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | |
High resolution limit [Å] | 1.800 * | |
Rmerge | 0.070 * | 0.320 * |
Number of reflections | 35211 | |
Completeness [%] | 80.0 * | 30 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 293 | PEG4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 16 (%) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |