1NCQ

The structure of HRV14 when complexed with pleconaril, an antiviral compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 3
Unit cell lengths	438.720, 438.720, 438.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.217
Rwork	0.216
R-free	0.21600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.540 *

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Rmerge	70.300 *	0.456 *
Total number of observations	1675367 *
Number of reflections	671838
Completeness [%]		26.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			The crystal was soaked in stabilization buffer containing 2ug/ml pleconaril before data collection

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.25 (M)
2	1	reservoir		0.25 (M)
3	1	reservoir		0.1 (M)
4	1	reservoir	PEG8000	0.3 (%)
5	1	drop	protein	20000 (mg/ml)