1NB8

Structure of the catalytic domain of USP7 (HAUSP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-04-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	75.647, 68.512, 76.261
Unit cell angles	90.00, 95.36, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.23
Rwork	0.222
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.510
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.057 *	0.370 *
Number of reflections	32867
Completeness [%]	96.4	93.3
Redundancy	3.54 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5 *	295	PEG 1000, Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	citrate	100 (mM)	pH5.5
3	1	reservoir	PEG3000	20 (%)