1NAP

THE CRYSTAL STRUCTURE OF RECOMBINANT HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-2 (M6L) AT 1.9-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	40.770, 43.810, 44.650
Unit cell angles	98.37, 120.28, 92.78

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.202
Rwork	0.202
R-free	0.23000
RMSD bond length	0.020
RMSD bond angle	0.038
Phasing software	X-PLOR
Refinement software	GPRLSA

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	7.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.025 *
Total number of observations	30511 *
Number of reflections	17830 *
Completeness [%]	97.0 *	47 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	PEG4000	28 (%)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir	ammonium acetate	200 (mM)