1NAF

Crystal structure of the human GGA1 GAT domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-15
Wavelength(s)	0.9796, 0.9798, 0.9393
Spacegroup name	P 63
Unit cell lengths	82.700, 82.700, 69.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.2484
Rwork	0.248
R-free	0.28300 *
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.180 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.066	0.399
Number of reflections	6684
<I/σ(I)>	14.2	2.6
Completeness [%]	99.7	99.7
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5	289	sodium acetate, lithium sulphate, NaH2PO4/K2HPO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	dithiothreitol	5 (mM)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.5
4	1	reservoir	sodium phosphate	0.3 (M)
5	1	reservoir	potassium phosphate	0.45 (M)
6	1	reservoir		0.2 (M)