1NA6

Crystal structure of restriction endonuclease EcoRII mutant R88A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	93
Detector technology	CCD
Collection date	2002-04-10
Detector	CUSTOM-MADE
Wavelength(s)	1.0088
Spacegroup name	P 1 21 1
Unit cell lengths	58.746, 92.360, 88.308
Unit cell angles	90.00, 108.08, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.236
Rwork	0.230
R-free	0.28400
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	ISIRAS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.053	0.387
Total number of observations	259113 *
Number of reflections	52027 *
<I/σ(I)>	11.5	3.8
Completeness [%]	99.6 *	99.9 *
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cacodylate	100 (mM)	pH6.3-6.5
2	1	reservoir	methanol	3.5-4 (%(v/v))
3	1	reservoir		35-40 (mM)