1N9R

Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	AREA DETECTOR
Wavelength(s)	0.9393
Spacegroup name	P 41 2 2
Unit cell lengths	79.891, 79.891, 251.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.25431
Rwork	0.254
R-free	0.26700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1I81 truncated to poly-serine
RMSD bond length	0.023
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.066	0.390
<I/σ(I)>	19.6	4.6
Completeness [%]	99.9	99.9 *
Redundancy	6.8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-NaCl
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	PEG3350	24 (%)
5	1	reservoir	Tris	0.1 (M)	pH8.5
6	1	reservoir	sodium acetate	0.1 (M)