1N9O

Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Composite data set.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.969
Spacegroup name	P 65 2 2
Unit cell lengths	121.900, 121.900, 45.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.252
Rwork	0.250
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g28
RMSD bond length	0.009
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.129 *	0.322 *
Number of reflections	5009
<I/σ(I)>	7.5	2.6
Completeness [%]	95.2	82.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.5 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)
3	1	drop		10 (mM)	pH8.0
4	1	reservoir	sodium HEPES	100 (mM)	pH7.3
5	1	reservoir	ammonium sulfate	1.5-1.8 (M)
6	1	reservoir	PEG8000	1-8 (%)