1N9L

Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in the dark state.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 65 2 2
Unit cell lengths	122.100, 122.100, 46.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.233
Rwork	0.232
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g28
RMSD bond length	0.008 *
RMSD bond angle	1.280 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.068 *	0.351 *
Total number of observations	77830 *
Number of reflections	16142
<I/σ(I)>	12.8	3.5
Completeness [%]	98.0	96.6
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.5 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)
3	1	drop		10 (mM)	pH8.0
4	1	reservoir	sodium HEPES	100 (mM)	pH7.3
5	1	reservoir	ammonium sulfate	1.5-1.8 (M)
6	1	reservoir	PEG8000	1-8 (%)