1N9G
Mitochondrial 2-enoyl thioester reductase Etr1p/Etr2p heterodimer from Candida tropicalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0760 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 229.453, 95.501, 164.229 |
Unit cell angles | 90.00, 124.71, 90.00 |
Refinement procedure
Resolution | 19.430 - 1.980 |
R-factor | 0.19731 |
Rwork | 0.195 |
R-free | 0.23480 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gu7 |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.460 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.430 * | 2.086 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.051 * | 0.348 * |
Number of reflections | 212894 | |
Completeness [%] | 97.5 * | 95.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | ammonium sulfate, ADA/NaOH, NADPH, octanoyl-CoA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
3 | 1 | reservoir | ADA | 0.1 (M) | pH7.0 |