1N8Q
LIPOXYGENASE IN COMPLEX WITH PROTOCATECHUIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.645, 136.961, 61.799 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ik3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.050 * |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (from CCP4) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 * | 0.450 * |
| Number of reflections | 52281 * | |
| <I/σ(I)> | 12 | 1.36 |
| Completeness [%] | 1.0 | 86.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 23 * | Skrzypczak-Jankun, E., (1997) Proteins, 29, 15. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | pH7. |
| 3 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 4 | 1 | reservoir | citrate-phosphate | 0.05 (M) | pH4.6 |
| 5 | 1 | reservoir | 0.2 (%(w/v)) | ||
| 6 | 1 | reservoir | sodium phosphate | 0.1 (M) | pH7. |
