1N81

Crystal structure of Pfg27 from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-13
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	58.880, 113.190, 91.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.100
R-factor	0.207
Rwork	0.207
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.011 *
RMSD bond angle	1.510 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	15.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.039	0.282
Number of reflections	18053
<I/σ(I)>	37.3	3.4
Completeness [%]	99.0	99
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	Hendrickson, W.A., (1990) EMBO J., 9, 1665. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	glucose	0.2 (%(w/v))
2	1	reservoir	carbenicillin	0.050 (mg/ml)