1N80
Bacteriophage T4 baseplate structural protein gp8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0723 |
Spacegroup name | P 1 |
Unit cell lengths | 66.869, 66.777, 82.158 |
Unit cell angles | 93.11, 101.28, 90.77 |
Refinement procedure
Resolution | 39.110 - 2.450 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n7z |
RMSD bond length | 0.008 |
RMSD bond angle | 1.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.053 | 0.176 |
Number of reflections | 49960 | |
<I/σ(I)> | 19.1 | 9.1 |
Completeness [%] | 97.7 | 82.4 |
Redundancy | 3.8 | 2.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293.15 | HEPES, PEG 6000, LiCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | reservoir | 1 (M) | ||
5 | 1 | reservoir | Zwittergent-3-10 | 12 (mM) | |
6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
7 | 1 | reservoir | PEG6000 | 16 (%) |