1N7V

THE RECEPTOR-BINDING PROTEIN P2 OF BACTERIOPHAGE PRD1: CRYSTAL FORM III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-01
Detector	BRANDEIS
Wavelength(s)	0.95
Spacegroup name	C 2 2 21
Unit cell lengths	46.500, 270.200, 137.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.110 - 2.200
R-factor	0.229
Rwork	0.229
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P2 Crystal Form I(1N7U)
RMSD bond length	0.006
RMSD bond angle	26.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.064 *	0.771 *
Number of reflections	38750
<I/σ(I)>	16.5
Completeness [%]	87.4	58.7 *
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	4 *	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	50 (%)
2	1	reservoir	sodium acetate	0.1 (M)	pH5.0
3	1	reservoir		10 (mM)