1N7E
Crystal structure of the sixth PDZ domain of GRIP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2000-09-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 40.267, 40.267, 222.872 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 * - 1.500 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.045 * | 0.360 |
Number of reflections | 151976 | |
<I/σ(I)> | 11.5 | 3.6 |
Completeness [%] | 98.3 | 93.3 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Park, S.H., (2002) Acta Cryst., D58, 1063. * |