1N5Q
Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with dehydrated Sancycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.240, 59.400, 72.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.740 |
| Rwork | 0.190 |
| R-free | 0.24000 |
| Structure solution method | fourier difference |
| Starting model (for MR) | 1lq9 |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 2.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.071 | 0.329 |
| Total number of observations | 118991 * | |
| Number of reflections | 21456 | |
| <I/σ(I)> | 21 | 3.8 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 293 | ammonium sulfate, PEG 200, Tris or Hepes buffer, pH 7.0, vapour diffusion + soaking, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | PEG200 | 10-15 (%) |
