1N5N

Crystal Structure of Peptide Deformylase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	90
Detector technology	CCD
Collection date	2002-01-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	68.612, 73.562, 75.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 1.800
Rwork	0.196
R-free	0.24500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bs4
RMSD bond length	0.044
RMSD bond angle	3.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.083 *	0.341 *
Number of reflections	36428
<I/σ(I)>	12
Completeness [%]	99.9 *	100
Redundancy	26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	20 *	Peg6000, LiCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	20 (%)
2	1	reservoir		1.0 (M)
3	1	reservoir	MES	0.1 (M)	pH6.0