1N54

Cap Binding Complex m7GpppG free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	200
Detector technology	CCD
Collection date	2002-01-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.943
Spacegroup name	P 32 2 1
Unit cell lengths	111.770, 111.770, 175.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 2.750 *
Rwork	0.263
R-free	0.29100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n52
RMSD bond length	0.011
RMSD bond angle	21.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.850
High resolution limit [Å]	2.750 *	2.690
Rmerge	0.061 *
Number of reflections	39454 *
<I/σ(I)>	20	2.3
Completeness [%]	100.0 *
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	4 *	Peg 400, Magnesium chloride, glycerol, Tris, glycine, ethylene glycol, pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG400	22 (%(w/v))
3	1	reservoir	Tris-HCl	100 (mM)	pH7.25
4	1	reservoir		125 (mM)
5	1	reservoir	glycerol	10 (%(v/v))
6	1	reservoir	dithiothreitol	2 (mM)
7	1	reservoir	glycine	10 (mM)
8	1	reservoir	ethylene glycol	3 (%(v/v))