1N51

Aminopeptidase P in complex with the inhibitor apstatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-24
Detector	RIGAKU RAXIS IIC
Spacegroup name	I 41 2 2
Unit cell lengths	139.319, 139.319, 231.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
Rwork	0.179
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1AZ9
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.280
High resolution limit [Å]	2.300 *	2.200
Rmerge	0.044 *	0.411 *
Number of reflections	55327
<I/σ(I)>	16.58	1.46
Completeness [%]	97.0 *	96.2 *
Redundancy	2.6 *	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	Magnesium Acetate, Sodium Cacodylate, MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.7 (mg/ml)
2	1	reservoir	magnesium acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.8
4	1	reservoir	MPD	25 (%)