1N2Z

2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 65
Unit cell lengths	133.066, 133.066, 67.687
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.189
Rwork	0.189
R-free	0.21000 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.320

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.058 *	0.182 *
Number of reflections	46346
Completeness [%]	100.0 *	100 *
Redundancy	10.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	VitaminB12	1 (mM)
2	1	drop	protein	15-20 (mg/ml)
3	1	reservoir	PEG400	30 (%)
4	1	reservoir	sodium acetate	0.1 (M)	pH4.6
5	1	reservoir		0.1 (M)