1N25
Crystal structure of the SV40 Large T antigen helicase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | SBC-2 |
| Wavelength(s) | 1.074385 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 120.321, 120.321, 132.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.243 |
| R-free | 0.27500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.379 * |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.125 | 0.544 |
| Total number of observations | 171257 * | |
| Number of reflections | 27610 | |
| <I/σ(I)> | 16.5 | 3.9 |
| Completeness [%] | 98.4 | 99.2 |
| Redundancy | 6.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | glycerol, DTT, Hepes, NaCl, EtOH, EDTA, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 10 | 1 | reservoir | EDTA | 1 (mM) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 7 | 1 | reservoir | HEPES | 50 (mM) | pH7.6 |
| 8 | 1 | reservoir | 105 (mM) | ||
| 9 | 1 | reservoir | ethanol | 5 (%) |
