1N1A
Crystal Structure of the N-terminal domain of human FKBP52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.805, 58.362, 70.904 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.204 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fkj |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.095 | 0.294 |
| Total number of observations | 26262 * | |
| Number of reflections | 8929 * | 889 * |
| <I/σ(I)> | 17.3 | 9.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.8 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Tris-HCl, Ammonium Sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 2 | 1 | drop | protein | 20-30 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
