1N0Y
Crystal Structure of Pb-bound Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.936, 30.792, 113.084 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 53.000 - 1.750 |
| R-factor | 0.21437 |
| Rwork | 0.213 |
| R-free | 0.22300 * |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.291 |
| Number of reflections | 33157 | |
| <I/σ(I)> | 14.6 | 4.1 |
| Completeness [%] | 98.1 | 94.7 |
| Redundancy | 5.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | MPD, sodium cacodylate, sodium acetate, lead nitrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 60-65 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH5.0 |
| 3 | 1 | drop | protein | 15 (mg/ml) |
