1N0R
4ANK: A designed ankyrin repeat protein with four identical consensus repeats
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2002-01-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.429, 46.115, 81.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.650 - 1.500 |
| R-factor | 0.21763 |
| Rwork | 0.216 |
| R-free | 0.24900 * |
| Structure solution method | Single anomolous diffraction |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.140 | |
| High resolution limit [Å] | 1.500 | |
| Rmerge | 0.070 * | 0.436 * |
| Number of reflections | 16966 | 913 * |
| Completeness [%] | 99.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | sodium cacodylate, magnesium bromide, MPD, isopropanol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.4 |
| 4 | 1 | reservoir | MPD | 33 (%) | |
| 5 | 1 | reservoir | isopropanol | 15 (%) |
