1N0Q
3ANK: A designed ankyrin repeat protein with three identical consensus repeats
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.636, 43.159, 105.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.260 |
| R-factor | 0.17218 |
| Rwork | 0.171 |
| R-free | 0.18700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ank |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.410 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | |
| High resolution limit [Å] | 1.260 | |
| Rmerge | 0.087 * | 0.080 * |
| Number of reflections | 41302 | 2246 * |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 3 | 1 | reservoir | MPD | 50 (%) | |
| 4 | 1 | drop | MPD | 30 (%) |
