1N00
Annexin Gh1 from cotton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 61.049, 61.049, 215.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.720 - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 20.900 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.043 * | 0.433 * |
| Total number of observations | 808320 * | |
| Number of reflections | 27412 | |
| Completeness [%] | 100.0 | 100 * |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 285 | ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0-1 (M) | ||
| 2 | 1 | drop | HEPES | 20 (%saturated) | pH8.0 |
| 3 | 1 | drop | 230-350 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
