1MZY
Crystal Structure of Nitrite Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.018 |
| Spacegroup name | H 3 |
| Unit cell lengths | 71.392, 71.392, 146.246 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.540 - 1.460 |
| Rwork | 0.185 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.540 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.204 | 0.410 |
| Number of reflections | 48257 | |
| <I/σ(I)> | 2.5 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 6000 or 4000, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
