1MZW

Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	44.369, 59.526, 47.040
Unit cell angles	90.00, 110.65, 90.00

Refinement procedure

Resolution	15.000 - 2.100 *
R-factor	0.195
Rwork	0.195
R-free	0.25700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qoi
RMSD bond length	0.005
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.100
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.063 *	0.236 *
Total number of observations	55492 *
Number of reflections	13762 *
Completeness [%]	99.2	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	21 *	PEG 8000, magnesium acetate, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	2 (%(w/v))
2	1	reservoir	magnesium acetate	200 (mM)
3	1	reservoir	HEPES	0.1 (M)	pH6.5