1MZV

Crystal Structure of Adenine Phosphoribosyltransferase (APRT) From Leishmania tarentolae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-14
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	64.538, 64.538, 234.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.270 - 2.200
R-factor	0.183
Rwork	0.181
R-free	0.21780 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qb7
RMSD bond length	0.014
RMSD bond angle	1.494
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.060 *
Total number of observations	229639 *
Number of reflections	14724 *
Completeness [%]	99.5	93.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	MOPS 100 mM, 22% isopropanol, Protein at 8 mg/ml, pH 7.00, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	AMP	2 (mM)
3	1	reservoir	MOPS	100 (mM)	pH7.0
4	1	reservoir	isopropanol	22 (%)