1MZV
Crystal Structure of Adenine Phosphoribosyltransferase (APRT) From Leishmania tarentolae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.538, 64.538, 234.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.270 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21780 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qb7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 * | |
| Total number of observations | 229639 * | |
| Number of reflections | 14724 * | |
| Completeness [%] | 99.5 | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | MOPS 100 mM, 22% isopropanol, Protein at 8 mg/ml, pH 7.00, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | AMP | 2 (mM) | |
| 3 | 1 | reservoir | MOPS | 100 (mM) | pH7.0 |
| 4 | 1 | reservoir | isopropanol | 22 (%) |
