1MZP

Structure of the L1 protuberance in the ribosome

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-28
Detector	MARRESEARCH
Wavelength(s)	0.9762,0.9330
Spacegroup name	P 65 2 2
Unit cell lengths	156.010, 156.010, 90.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.650
R-factor	0.219
Rwork	0.219
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	20.600 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.057	0.376
Total number of observations	277313 *
Number of reflections	35675 *
Completeness [%]	99.1	97.2
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6 *	4 *	PEG400, Tris HCl, KCl, MgCl2, ph 8.5, VAPOR DIFFUSION, HANGING DROP at 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	Tris HCl
3	1	1	KCl
4	1	1	MgCl2
5	1	2	PEG 400
6	1	2	KCl
7	1	2	MgCl2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG400	10 (%(v/v))
10	1	reservoir		100 (mM)
11	1	reservoir		10 (mM)
2	1	drop		50 (mM)	pH8.6
3	1	drop		100 (mM)
4	1	drop		10 (mM)
5	1	drop	glycerol	4 (%(v/v))
6	1	drop	RNA	3 (mg/ml)
7	1	drop	SacL1	4.8 (mg/ml)
8	1	reservoir	PEG400	27 (%(v/v))
9	1	reservoir	Tris-HCl	50 (mM)	pH8.5