1MZF
Human Factor inhibiting HIF (FIH1) in Complex with 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.460, 86.460, 147.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.400 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FIH-1 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.280 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 * | 0.443 * |
| Total number of observations | 172309 * | |
| Number of reflections | 21793 * | |
| <I/σ(I)> | 21.8 | 5.1 |
| Completeness [%] | 98.2 * | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 21 * | potassium tartrate, CAPSO, iron(II) sulfate, 2-oxoglutarate, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 5 (mM) | |
| 5 | 1 | reservoir | CAPSO | 50 (mM) | pH9.6 |
| 6 | 1 | reservoir | potassium tartrate | 0.9-1.2 (M) | |
| 7 | 1 | reservoir | glycerol | 30 (%) | |
| 8 | 1 | reservoir | 5 (mM) | ||
| 9 | 1 | reservoir | 2-OG | 20 (mM) |
