1MZB

Ferric uptake regulator

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-20
Detector	MARRESEARCH
Wavelength(s)	1.2837, 1.2828, 1.1310
Spacegroup name	I 41 2 2
Unit cell lengths	63.300, 63.300, 180.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.800
Rwork	0.226
R-free	0.25400 *
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.490 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.049 *	0.425 *
Total number of observations	223710 *
Number of reflections	17481 *
Completeness [%]	99.8 *	99.9
Redundancy	12.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethylene glycol	40 (%)
2	1	reservoir	Tris	100 (mM)	pH7.0
3	1	reservoir		10 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	drop	Tris	10 (mM)	pH7.5
6	1	drop		100 (mM)
7	1	drop	glycerol	10 (%)
8	1	drop	ascorbic acid	1 (mM)