1MYK

CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	51.800, 51.800, 110.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.217
Rwork	0.217
R-free	0.27700
RMSD bond length	0.013
RMSD bond angle	1.490
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.094 *
Number of reflections	5340
Completeness [%]	90.4	56.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	bis-tris propane-HCl	30 (mM)
3	1	drop		1 (mM)
4	1	drop	PEG3400	22-24 (%)	precipitant
5	1	drop	2-propanol	7-9 (%)	precipitant
6	1	drop	NaHEPES	100 (mM)	precipitant
7	1	reservoir	precipitant