1MX2
Structure of F71N mutant of p18INK4c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.344, 151.115, 40.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.218 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.250 | 2.000 |
| Rmerge | 0.085 | 0.496 |
| Number of reflections | 22638 | |
| <I/σ(I)> | 15.2 | 2.7 |
| Completeness [%] | 92.7 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 273 | 20 mM Tris, 25mM (NH4)2HPO4, 0.5 mM DTT, 7% PEG 6000, 2 M NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.5 |
| 3 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 4 | 1 | drop | PEG6000 | 7 (%) | |
| 5 | 1 | drop | 1 (M) | ||
| 6 | 1 | reservoir | PEG6000 | 14 (%) | |
| 7 | 1 | reservoir | 2 (M) |
