1MWM

ParM from plasmid R1 ADP form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9600
Spacegroup name	P 41
Unit cell lengths	64.710, 64.710, 199.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.000
R-factor	0.2224
Rwork	0.221
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Semet ParM
RMSD bond length	0.008 *
RMSD bond angle	1.381 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	21.900
High resolution limit [Å]	2.000 *
Rmerge	0.064 *
Redundancy	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	4 *	100mM Na-cacodyltae, 400mM NaCl, 400mM Mg-acetate, 11% PEG8000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium cacodylate	100 (mM)	pH6.2
2	1	reservoir		400 (mM)
3	1	reservoir	magnesium acetate	400 (mM)
4	1	reservoir	PEG8000	11 (%)
5	1	drop	protein	10 (mg/ml)
6	1	drop	ADP	1 (mM)
7	1	drop		1 (mM)