1MWL

Crystal structure of geneticin bound to the eubacterial 16S rRNA A site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 1 21 1
Unit cell lengths	46.200, 33.180, 51.740
Unit cell angles	90.00, 104.13, 90.00

Refinement procedure

Resolution	25.000 * - 2.400
R-factor	0.224
Rwork	0.224
R-free	0.25000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LC4 (RNA ONLY)
RMSD bond length	0.007
RMSD bond angle	10.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.062 *	0.211 *
Total number of observations	24145 *
Number of reflections	5466	566 *
<I/σ(I)>	39.3	6.7
Completeness [%]	88.8	92.8
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	37 *	MPD, MAGNESIUM SULPHATE, POTASSIUM CHLORIDE, SODIUM CHLORIDE, SODIUM CACODYLATE, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K
1	VAPOR DIFFUSION, HANGING DROP	6.4	37 *	MPD, MAGNESIUM SULPHATE, POTASSIUM CHLORIDE, SODIUM CHLORIDE, SODIUM CACODYLATE, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MPD	1.5-3.5 (%)
2	1	drop	glycerol	1.0-2.0 (%(v/v))
3	1	drop	geneticin	2 (mM)
4	1	drop		150 (mM)
5	1	drop	sodium cacodylate	50 (mM)	pH6.4
6	1	reservoir	MPD	40 (%)