1MWJ
Crystal Structure of a MUG-DNA pseudo substrate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.488 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 101.996, 101.996, 45.133 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.850 |
R-factor | 0.23 |
Rwork | 0.190 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Native MuG |
RMSD bond length | 0.010 |
RMSD bond angle | 24.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.087 | 0.278 * |
Number of reflections | 5479 * | |
<I/σ(I)> | 9.7 | 2 |
Completeness [%] | 94.5 | 96.7 * |
Redundancy | 2.3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 4.6 | 289 | Barrett, T.E., (1998) Cell., 92, 117. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | Sodium Acetate | ||
3 | 1 | 2 | Sodium Acetate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
2 | 1 | Tris-HCl | 20 (mM) | pH8.0 | |
3 | 1 | PEG4000 | 8 (%) | ||
4 | 1 | 2 | sodium acetate | 0.1 (M) | pH4.6 |