1MVY
Amylosucrase mutant E328Q co-crystallized with maltoheptaose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2000-03-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.016 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.028, 116.358, 60.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.310 - 2.000 |
| Rwork | 0.181 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.300 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 * | 0.220 * |
| Total number of observations | 413622 * | |
| Number of reflections | 45487 | |
| Completeness [%] | 97.9 * | 95.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Skov, L.K., (2000) Acta Crystallogr., D56, 203. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-6 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | alpha-dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
