1MVO

Crystal structure of the PhoP receiver domain from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-04
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 41 21 2
Unit cell lengths	45.704, 45.704, 134.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.100 * - 1.600
R-factor	0.188
Rwork	0.186
R-free	0.23300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Metal-free PhoPN structure solved by MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.100 *	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.091	0.187
Total number of observations	224913 *
Number of reflections	18249 *
<I/σ(I)>	5.2	3.5
Completeness [%]	92.0 *	92
Redundancy	6.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8 *	4 *	PEG 10000, sodium citrate, PEG MME 550, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG10000	23-25 (%)
2	1	reservoir	sodium citrate	0.1 (M)	pH5.8
3	1	reservoir	PEG550 MME	3 (%)
4	1	drop	Tris	20 (mM)	pH7.8
5	1	drop		150 (mM)
6	1	drop	EDTA	0.1 (mM)
7	1	drop	dithiothreitol	1 (mM)