1MVF

MazE addiction antidote

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-21
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	P 1 21 1
Unit cell lengths	29.440, 47.826, 128.376
Unit cell angles	90.00, 90.59, 90.00

Refinement procedure

Resolution	15.000 - 1.650
R-factor	0.2203
Rwork	0.218
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.750
High resolution limit [Å]	1.650	1.650
Rmerge	0.070	0.228 *
Total number of observations	96388 *
Number of reflections	32375 *	2633 *
<I/σ(I)>	11.82
Completeness [%]	74.1	61 *
Redundancy	2.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	293	PEG 8000, magnesium acetate, cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	Vapor diffusion *	6.5	293	PEG 8000, magnesium acetate, cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5
4	1	reservoir	magnesium acetate	0.2 (M)