1MUJ
Crystal structure of murine class II MHC I-Ab in complex with a human CLIP peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-17 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.647, 88.065, 83.740 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 33.060 - 2.150 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.060 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.038 * | 0.540 * |
| Number of reflections | 24657 | |
| <I/σ(I)> | 24.1 | 1.65 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 1.87 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 22 * | PEG3000, Sodium Chloride, Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 100 (mM) | pH7.5 |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | PEG3000 | 22.5 (%) | |
| 4 | 1 | reservoir | MPD | 5 (%(v/v)) | |
| 5 | 1 | drop | protein | 5-10 (mg/ml) |
