1MUF
Structure of histone H3 K4-specific methyltransferase SET7/9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-18 |
| Detector | SBC-2 |
| Wavelength(s) | 0.95372,0.9791,0.97921 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 110.973, 110.973, 96.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.220 - 2.260 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.23000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.032 * |
| RMSD bond angle | 2.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.096 * | |
| Number of reflections | 30900 * | 3074 * |
| Completeness [%] | 100.0 * | 100 * |
| Redundancy | 10.7 * | 8.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | PEG 3000, CaCl2, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | PEG3000 | 19 (%(w/v)) |
