1MU0
Crystal Structure of the Tricorn Interacting Factor F1 Complex with PCK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.579, 61.721, 80.435 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.366 |
| Rwork | 0.314 |
| R-free | 0.36600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mt3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.948 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 * |
| Number of reflections | 11344 * |
| <I/σ(I)> | 3.2 |
| Completeness [%] | 85.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 293 | 12% PEG 6000, 100 mM Bis-Tris-HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | Bis-Tris-HCl | 100 (mM) | pH6.0 |
| 4 | 1 | reservoir | PEG6000 | 7-12 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG8000 | 20 (%) |
