1MT1
The Crystal Structure of Pyruvoyl-dependent Arginine Decarboxylase from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.770, 92.990, 87.230 |
| Unit cell angles | 90.00, 94.84, 90.00 |
Refinement procedure
| Resolution | 63.500 - 2.200 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 2.240 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.142 * | 0.383 * |
| Total number of observations | 368924 * | |
| Number of reflections | 52185 * | |
| <I/σ(I)> | 4.1 | 1.6 |
| Completeness [%] | 100.0 * | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000, 2-methyl-2,4-pentanediol, glycerol, n-[2-hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid], beta octyl glucoside, putrescine, ethylenediaminetetraacetic acid dithiothreitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 18-21 (%) | |
| 2 | 1 | reservoir | MPD | 10 (%) | |
| 3 | 1 | reservoir | glycerol | 2.5 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 5 | 1 | reservoir | beta-octylglucoside | 0.5 (mM) | |
| 6 | 1 | reservoir | putrescine | 0.5 (mM) | |
| 7 | 1 | reservoir | EDTA | 5 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 10 (mM) |
