1MSV
The S68A S-adenosylmethionine decarboxylase proenzyme processing mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.936, 56.382, 99.190 |
| Unit cell angles | 90.00, 110.97, 90.00 |
Refinement procedure
| Resolution | 99.000 * - 1.750 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i76 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.250 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.117 * | 0.280 * |
| Total number of observations | 551036 * | |
| Number of reflections | 82984 * | 11752 * |
| <I/σ(I)> | 3.9 | 1.8 |
| Completeness [%] | 98.7 | 96.3 |
| Redundancy | 6.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 8000, tris[hydroxymethyl]aminomethane, dithiothreitol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 14-16 (%(w/w)) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
