1MRI

STUDIES ON CRYSTAL STRUCTURES ACTIVE CENTER GEOMETRY AND DEPURINE MECHANISM OF TWO RIBOSOME-INACTIVATING PROTEINS

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	130.970, 130.970, 40.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.192
Rwork	0.192
RMSD bond length	0.012
RMSD bond angle	1.450
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.062 *
Number of reflections	11731 *
Completeness [%]	83.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%(w/v))
2	1	reservoir	PEG6000	14 (%(w/v))
3	1	reservoir	sodium citrate	0.3 (M)